KYSE-30HUMAN ASIAN SQUAMOUS CELL CARCINOMA (C15-1314-110)

Sigma

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Was: $2,039.50
Now: $1,835.55
SKU:
C005B-237994
UPC:
51113103
Condition:
New
Availability:
3 Days
HazmatClass:
No
ManufacturerName:
Sigma-Aldrich
QTY1:
1
UOM1:
EA
WeightUOM:
LB
ShortDescription:

Cell Line Origin

Human Asian squamous cell carcinoma

Cell Line Description

KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well d

MPN:
824-94072011-1VL
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Product Overview

Cell Line Origin

Human Asian squamous cell carcinoma

Cell Line Description

KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well differentiated squamous cell carcinoma. The cell line KYSE-30 was established with the use of tumours initially transplanted to athymic mice. The cells are reported to have a doubling time of 20.8 hrs in the exponential growth phase. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2x10,000,000 sites/cell.

Application

Study of human oesophageal cancer.

Culture Medium

RPMI 1640 + Ham′s F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: human esophagus. growth mode: Adherent. karyotype: Aneuploid. morphology: Polygonal. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196C.

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